When Yoshi Circuit returned as a retro track in Mario Kart DS, the Ribbon signs returned as well, making this Birdo's only appearance in the game. On the Mushroom City and Yoshi Circuit courses, there are signs that read 'Ribbon' with 'Birdo watching' in smaller letters near the center, a pun on the observation term birdwatching. Birdo's bow is smaller in this game than in regular appearances. This staff ghost also features the same characters on the Yoshi Circuit course. Her signature emblem appears in the Dry Dry Desert course, and the staff ghost features her and Yoshi riding the Turbo Yoshi. Her unlockable kart is the Turbo Birdo, a middleweight kart with average speed and acceleration, while being slightly heavier than the other middleweight karts. Her special item is the Birdo's Egg which functions like the Yoshi's Egg, but has pink spots and is vastly different from Birdo's pure white eggs. When the second title screen is unlocked, Birdo can also team up with Peach. In this game, she is Yoshi's default partner.
Birdo's first appearance in the Mario Kart series is Mario Kart: Double Dash!!.
